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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Semin Cancer Biol. 2015 Aug 1;35:191–199. doi: 10.1016/j.semcancer.2015.07.002

Figure 1.

Figure 1

Schematic illustration of strategies to enrich cell populations with varying sizes. (A) Single cells from normal or cancerous cultures or tissues can be separated into (relatively) small, intermediate and large cell populations based on the forward scatter (FSC) intensity value in flow cytometry (i.e., FSC-based FACS). (B) Size-sieving approach using nylon mesh filtration. Single cells are separated into small cells (<10 µm) and large cells (≥ 20 or 30 µm) using 20 or 30 µm nylon mesh. (C–D) Cell populations with different sizes can be more precisely purified via a beads-sizing method by FACS (i.e., beads sizing-based FACS) (modified from Figure 1-A of ref. 32).