Skip to main content
. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Semin Cancer Biol. 2015 Aug 1;35:191–199. doi: 10.1016/j.semcancer.2015.07.002

Figure 2.

Figure 2

Small PC3 cells tend to be more clonal and tumorigenic than the isogenic large cells. (A) FSC-based FACS-purified small PC3-GFP cells exhibited a trend of increasing tumorigenicity than isogenic large cells. (B–E) PC3 cells were separated by size-sieving using nylon mesh into three (small, intermediate, and large) sizes and then used in various in vitro and in vivo assays. (B) Morphologic validation and GFP checking of small (<10 µm) and corresponding large (≥30 µm) cells. (C) Clonal assays. PC3-GFP cells of two different sizes were plated in 6-well plates (300 cells per well). Clones were counted 14 days after plating. Presented are the mean ± SD from triplicate wells (*P<0.05). (D–E) Two independent tumor experiments. PC3-GFP cells were separated by nylon mesh into the 3 sizes indicated and subcutaneously injected into NOD/SCID female mice at different cell doses. Presented are tumor images, tumor incidence, and the time (days; bottom) when tumors were harvested.