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Emission spectra of BSA-hydrazine and BSA reacted with or without probe 6 excited at 320 nm. BSA or BSA-hydrazine (final concentration 25 μM) was allowed to react with or without probe 6 (final concentration 75 μM) at room temperature for 15 min. Excess probe was removed by rapid gel filtration chromatography and emission spectra of equal concentration of each protein sample were obtained.
