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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2015 Oct 3;289(2):155–162. doi: 10.1016/j.taap.2015.09.029

Figure 1. Stoke’s shift of HSA-prodan complex yields fluorescent maxima at 465 nm.

Figure 1

HSA was incubated with a 10-fold excess of prodan and allowed to equilibrate for 30 minutes. Unbound prodan was removed by acetone precipitation and the emission spectra of free prodan, HSA-prodan and HSA were obtained. Excitation λ is 380 nm and upon binding HSA, prodan λmax blue-shifts from 520 nm to 465 nm. HSA without prodan (bottom spectra) shows negligible spectral emission. The endpoint values to monitor prodan displacement from the HSA-prodan complex are 380 nm excitation, 465 nm emission.