Table 1.
R257 peptide MRM transitions
| R257 Peptide Sequence | Parent | Daughter | CE | Charge | Ion |
|---|---|---|---|---|---|
| VHTEC(57)C(57)HGDLLEC(57)ADDR(54)ADLAK | 660.6 | 801.5 | 31 | 4 | y20+3 |
| 660.6 | 635.6 | 32 | 4 | y21+4 | |
| 660.6 | 237.1 | 35 | 4 | b2 | |
| 660.6 | 109.6 | 90 | 4 | y2+2 | |
| 660.6 | 421.5 | 33 | 4 | y7+2 | |
| 528.7 | 605.2 | 19 | 5 | b10+2 | |
| 528.7 | 594.7 | 19 | 5 | y10+2 | |
| 528.7 | 659.3 | 19 | 5 | y11+2 | |
| 528.7 | 661.7 | 19 | 5 | b11+2 |
Nine transitions were generated for peptide R257 by modifying a pure peptide with MG. The transitions in bold were the transitions selected for further use in analysis, as they had the best linear response to concentration changes in vitro by MG. CE: collision energy.