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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: J Struct Biol. 2015 Sep 28;192(3):342–348. doi: 10.1016/j.jsb.2015.09.012

Figure 1. Analyses of PIP3 binding to LRH-1 LBD.

Figure 1

(A) Determination of binding affinity of PIP3 for LRH-1 by EMSA; data are shown as average of four (n=4) independent measurements, with experimental errors indicated. (B) DSF assay shows stabilization of LRH-1 upon PIP3 and DAX-1 peptide binding. Melting temperatures (Tm) of the Apo-, bPL- and PIP3-bound LRH-1 are shown as average of three (n=3) independent measurements.