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. Author manuscript; available in PMC: 2016 Nov 17.
Published in final edited form as: Circulation. 2015 Sep 10;132(20):1880–1890. doi: 10.1161/CIRCULATIONAHA.115.016160

Figure 2.

Figure 2

IL-1β drives HSC proliferation directly. (a) Flow cytometric gating for different bone marrow (BM) stromal cell populations (EC, bone marrow endothelial cells; MSC, bone marrow mesenchymal stromal cells; OB, bone osteoblastic lineage cells). Quantification of interleukin-1 receptor 1 (IL-1R1) expression on hematopoietic (HSC and Lin− sca-1+ c-kit+ cells (LSK)) and stromal (EC, MSC and OB) bone marrow cells by qRT-PCR (n = 4–5 per group). (b) Lethally irradiated wild type (WT) mice were reconstituted with either wild type or IL1R1−/− bone marrow. Four months later, mice received MI and HSC proliferation was quantified 48h later (n = 8–9 per group, mean ± SD, *p<0.05, **p<0.01, ****p<0.0001, one-way ANOVA).