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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Cancer Chemother Pharmacol. 2015 Oct 29;76(6):1297–1307. doi: 10.1007/s00280-015-2879-4

Figure 5. NAC and GEE do not interfere with anthracycline cytotoxicity in acute leukemia cells. NAC protects H9C2 cells from anthracycline cytotoxicity. NAC increases GSH levels in acute leukemia cells treated with anthracyclines.

Figure 5

A) Jurkat and B) ML-1 cells were pretreated with NAC and GEE and then exposed to DOX and DNR for 24 hours. Viability was assessed. C) H9C2 cells were pretreated with NAC and subsequently exposed to DOX and DNR for 24 hours and DNA fragmentation was assessed. The results represent the mean ± SEM of 3 different experiments D, E) Jurkat and F, G) ML-1 cells were pretreated with N-acetylcysteine (NAC), and then exposed to DOX and DNR. GSH levels were measured by quantification of fluorescence by spectrofluorometry. The results represent the mean ± SEM of 3 different experiments, *p<0.05, **p<0.01.