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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: DNA Repair (Amst). 2015 Oct 23;35:126–136. doi: 10.1016/j.dnarep.2015.10.003

Figure 3. Effect of MLH1 knockdown on cisplatin cytotoxicity and repair.

Figure 3

(A) Colony survival assays in WT and Polβ deficient MDA-MB-231 cells with MLH1 KD. (B) ELISA for cisplatin GG intrastrand adduct repair in MLH1 KD MDA-MB-231 cells. Cells were treated with cisplatin for 2 hrs and genomic DNA was extracted at the indicated time points. ELISA was performed using an antibody specific for cisplatin GG intrastrand adduct and the percentage of GG adducts remaining was calculated as described. Results are represented as mean ± SD of three independent experiments. Statistical analysis was performed by student’s t test and comparisons are made between wildtype and proficient cells vs deficient cells. NS – non-significant (C) Cisplatin ICL DNA repair using alkaline comet assay performed in WT and Pol β deficient MDA-MB231 cells as described previously. (D) Immunofluorescence assay for γH2AX assessing DSB repair in WT, MLH1 KD and Polβ deficient MDA-MB-231 cells.