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. Author manuscript; available in PMC: 2016 Aug 19.
Published in final edited form as: Bioconjug Chem. 2015 Jun 22;26(8):1633–1639. doi: 10.1021/acs.bioconjchem.5b00241

Figure 2.

Figure 2

Synthesis and characterization of pyro-s-PEI. A) Reaction of s-PEI with pyro. B) Absorption spectra of labeled PEI and s-PEI samples in water following labeling and purification. C) Fluorescence emission of pyro-labeled samples in methanol, normalized to free pyro in methanol. All samples had the equal absorption values at the excitation wavelength of 410 nm. D) Fluorescence emission of pyro-labeled PEI and s-PEI samples in water, normalized to the maximum emission intensity of free pyro (which was measured in methanol since it has limited water solubility). E) Barium chloride turbidity assay for sulfate detection. F) Blood clotting time of freshly drawn mouse blood immediately incubated with indicated samples (for PEI samples, the final concentration was 100 μg/mL). Mean +/− std. dev. for triplicate measurements.