Fig. 2.

Global liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOFMS) metabolomics. Cloud plots generated by XCMS Online showing dysregulated features between control and PDR samples (two-tailed Mann–Whitney test) for a RPLC–MS analysis, control (n = 11), PDR (n = 9) top plot and b HILIC–MS analysis, control (n = 11), PDR (n = 7), lower plot. Total ion chromatograms (TICs) for each sample can be seen on the plot; features whose intensity are increased in PDR vitreous are shown on the upper part of the plot as blue circles and features whose intensity decreases are shown on the bottom part of the plot as green circles. Larger and brighter circles (features) correspond to larger fold changes and lower p-values respectively