Fig. 3.

Analyses of purified AMs from healthy versus diseased lungs by quantitative real-time RT-PCR. a AMs from erythrocyte-depleted BAL cell suspensions were purified by adhesion (90 min, 37 °C) on bacteriological plastic, detached by trypsin–EDTA treatment, and purity was analyzed by FACS. Left square exemplarily depicts percentages of AMs (CD11c^high, F4-80^high) of analyzed singlets in non-purified BAL samples from SPC-HAxTCR-HA mice. Right square depicts AM percentages of BAL cells from SPC-HAxTCR-HA mice after purification. Note the decreased abundance of the surface molecule CD11c on AMs after trypsin–EDTA treatment. b Purified alveolar macrophages from SPC-HA and SPC-HA xTCR-HA mice were pooled (n = 3–5 mice/group), RNA was extracted, and relative expression of indicated transcripts was determined. Gene expression was normalized to the SPC-HA control group