Table 4.
Naphthalene-utilizing bacterial isolates extracted from the sediment and their identification based on MALDI-TOF MS with MALDI Biotyper and/or 16S rRNA gene analysis.
| Isolate designation | MALDI Biotyper-based identification | Scorea | Closest RDP SeqMatch type strain; similarity scoreb | Accession no. of the type strain | GenBank accession no. | |
|---|---|---|---|---|---|---|
| 16S rRNA | npahc | |||||
| 20a2 | Pseudomonas veronii | +++ | Pseudomonas veronii CIP 104663; 1.000 | AF064460 | KJ643424 | KJ643430 |
| 10a3 | Ps. gessardii | ++ | Pseudomonas gessardii CIP 105469; 1.000 | AF074384 | KJ643425 | KJ643431 |
| 20b3 | No reliable identificaiton | – | Pseudomonas taiwanensis BCRC 17751; 0.992 | EU103629 | KJ643426 | KJ643432 |
| 20a3 | Comamonas testosteroni | +++ | Comamonas testosteroni ATCC 11996; 0.998 | M11224 | KJ643427 | KJ643433 |
ND, not detected.
a
Scores: +++, highly probable species identification; ++, probable species identification; +, probable genus identification; –, no reliable identification.
b
Similarity reports the percent sequence identity over all pairwise comparable positions.
c
Naphthalene dioxygenase gene.