Fig. 1. .

Evaluation of coated glass slides optimal for immunoelectron microscopy. (a) Results of transfer of immunostained paraffin sections mounted on the coated glass slides to epoxy resin (EPON). Five sections were evaluated under each condition. The vertical row represents Silane S, Silane, New Silane II and New Silane III slides in the upper panels, and APS, PLL, MAS and TACAS slides in the lower panels. Sections, particularly located in the center of the circle rimmed by the gelatin capsule, often remain on the glass slides, and often show brown color (burnt by heat). No sections remain on TACAS slides, except for one (arrow). Successfully transferred cases are framed in blue. (b) A section mounted on a Silane S slide after trial of tissue transfer. Tissue section mostly remains on the glass slide, and the surface of EPON block appears to be irregular and rough. (c) A section mounted on a TACAS slide after trial of tissue transfer. No tissue section remains on the glass slide, and the EPON block is smooth-surfaced.