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. 2015 Nov 18;197(24):3760–3768. doi: 10.1128/JB.00521-15

FIG 3.

FIG 3

(A) Six-hundred-megahertz COSY NMR spectrum of WbwAO104 product sialylα2-3Galβ1-3GalNAcα-PO3-PO3-(CH2)11-O-phenyl. WbwA product (structure shown) was isolated by HPLC as described in Materials and Methods, dissolved in D2O, and analyzed by 1D and 2D experiments. The axial H-3 of sialic acid appears to be close in space to the axial H-3 of Gal. (B) Six-hundred-megahertz NOESY NMR spectrum of WbwAO104 product sialylα2-3Galβ1-3GalNAcα-PO3-PO3-(CH2)11-O-phenyl. The axial H-3 of sialic acid shows nuclear Overhauser effects (NOEs) to the equatorial H-3 of sialic acid and to the H-3 of Gal. (C) Six-hundred-megahertz ROESY NMR spectrum of WbwAO104 product sialylα2-3Galβ1-3GalNAcα-PO3-PO3-(CH2)11-O-phenyl. As in the NOESY spectrum, the axial H-3 of sialic acid shows NOEs to the equatorial H-3 of sialic acid and to the H-3 of Gal.