TABLE 2.
Main characteristics of BKV DNA quantification assays
| Parameter | Value or description |
||
|---|---|---|---|
| R-gene | GeneProof | RealStar | |
| Target | t-Ag | VP1/VP2 | T-Ag and t-Ag |
| Fluorescence label on probe | FAM | FAM | FAM |
| No. of PCR cycle steps (cycle steps) | 2 (95°C, 60°C) | 3 (95°C, 60°C, 72°C) | 2 (95°C, 58°C) |
| Final vol (μl) of assay mixture | 25 | 40 | 30 |
| Volume (μl) of extracted DNA | 10 | 10 | 10 |
| No. of quantification standards | 4 | 4 | 4 |
| Range of standard curve (copies/ml) | 1,250–1,250,000 | 2,500–2,500,000 | 2,500–2,500,000 |
| Quantification range (log copies/ml) | 2.14–11.0 | 2.17–7.0 | 2.25–9.0 |
| Simultaneous amplification of internal control | Yes | Yes | Yes |