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Bilirubin inhibits DSS-induced colonic inducible nitric oxide synthase (iNOS) expression. Immunohistochemical staining for iNOS was performed on frozen sections of colon tissue obtained from mice treated with vehicle alone (top left), vehicle plus DSS (top middle), bilirubin alone (bottom left), or bilirubin plus DSS (lower middle). Representative low-power (×200) images are shown. Right: specimens stained with an isotype antibody (iso) as control.
