Fig. 3.

Bilirubin inhibits eosinophil infiltration and VCAM-1 expression in the colon of DSS-treated mice. Top: representative high-power (×400) images of 2-cm sections of distal colon obtained from DSS-treated mice administered vehicle (left) or bilirubin (right) and stained with Sirius red to highlight eosinophils (arrows). Middle: results of immunohistochemical staining of proximal colon specimens for VCAM-1 (×200). Bottom: graph of mean number of eosinophils (±SE) per high-power field (hpf) averaged over 10 separate fields (n = 9–10 per treatment group). *P < 0.001 vs. vehicle plus DSS.