Differences in metabolic hormones in fasting and postprandial conditions between PAC1−/− and WT mice. In fasting conditions, mice were fasted overnight and plasma was withdrawn. In postprandial conditions, mice were fasted overnight and refed for 30 min before plasma was withdrawn. Plasma levels of active ghrelin (A), percentage of active ghrelin/total ghrelin (B), GLP-1 (C), insulin (D), leptin (E), glucagon (F), and PYY (G) were assessed in PAC1−/− compared with WT mice. Data are means ± SE of 8 mice/group. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.