Fig. 2.
Representative light photomicrographs demonstrating either the presence or absence of resistin immunolocalization within either the lung parenchyma (LP) and adjacent adipose tissue (AT) (A−D) or airways (bronchioles) (E and F) of wild-type C57BL/6 (A, B, D, E, and F) or resistin-deficient mice (C) 24 h following cessation of a 3-h exposure to either filtered room air (air) or ozone (O3; 2 ppm). Tissue sections in A, B, C, E, and F were incubated with an anti-mouse resistin-antibody while the tissue section in D was incubated with a polyclonal goat immunoglobulin (IgG), an isotype control. The lungs were fixed in situ with 10% phosphate-buffered formalin 24 h following the cessation of exposure to air or O3. In A−F, the images have been magnified with a 40× objective lens while each of the scale bars in A−F represents 50 μm; n = 6 mice in each group.