Fig. 3.

Toll-like receptor 4 (TLR4) signaling is not required for HDE-mediated conditioning of lung cDCs. CD103⁺ cDCs (A) or CD11b^hi cDCs (B) were prepared from the lungs of HDE/OVA-treated wild-type (WT, open bars) or Tlr4^−/− mice (solid bars) and cultured with naïve CD4⁺ OT-II cells to induce Th cell differentiation. Bars represent the means ± SD of duplicate wells. Data are from 1 experiment, representative of 2 experiments. *P < 0.05, **P < 0.01.