Fig. 4.

HDE exposure indirectly conditions cDCs to induce Th2 responses. A: CD103⁺ cDCs were prepared from the lungs of untreated mice and cultured with either media alone, bronchoalveolar lavage fluid (BALF) from PBS-treated (BALF-PBS) or HDE-treated (BALF-HDE) mice, or HDE (1% vol/vol). After 24 h, DCs were washed and cultured with naïve CD4⁺ OT-II cells in the presence of OVA peptide to induce Th cell differentiation. Bars represent the means ± SD of duplicate wells. Data are from 1 experiment, representative of 2 experiments. B: FLT3L-derived cDCs (FL-cDCs) were treated as described in A and cultured with naïve CD4⁺ OT-II cells in the presence of OVA peptide to induce Th cell differentiation. Bars represent the means ± SD of duplicate wells. Data are from 1 experiment, representative of 3 experiments. *P < 0.05, **P < 0.01.