Fig. 6.
Activation of cDCs with BALF-HDE results in upregulation of CD80 but low expression of CD40, CD86, and IL-12p40. A–C: FL-cDCs were treated for 24 h with either media, HDE (1% vol/vol), BALF-PBS, or BALF-HDE. A: representative histograms of I-Ab (MHC II) and costimulatory molecule display on FL-cDCs following the indicated treatments. B: MFI of I-Ab or costimulatory molecule expression as determined by flow cytometry. Bars represent the means ± SD of triplicate samples. Data are from 1 experiment, representative of 3 experiments. C: Il12b mRNA expression in treated FL-cDCs was measured by qPCR and normalized to Gapdh expression. Bars represent the means ± SD of duplicate samples. Data are from 1 experiment, representative of 2 experiments. **P < 0.01, ***P < 0.001. AU, arbitrary units.