Table 1.
Normalized cAMP Reading |
||
---|---|---|
cNMP Added, pmol | 4 pmol cAMP | 150 pmol cAMP |
0 cNMP | 1.00 ± 0.00 | 1.00 ± 0.00 |
15 cGMP | 1.37 ± 0.49 | 1.05 ± 0.08 |
150 cGMP | 1.48 ± 0.02 | 1.06 ± 0.08 |
1,500 cGMP | 4.3 ± 0.1 | 1.23 ± 0.14 |
15,000 cGMP | 9.6 ± 0.3 | 1.71 ± 0.26 |
15 cCMP | 0.88 ± 0.07 | 1.10 ± 0.10 |
150 cCMP | 0.73 ± 0.19 | 1.04 ± 0.12 |
1,500 cCMP | 1.70 ± 0.28 | 1.08 ± 0.11 |
15,000 cCMP | 5.3 ± 0.4 | 1.36 ± 0.15 |
15 cUMP | 0.99 ± 0.14 | 1.39 ± 0.31 |
150 cUMP | 1.21 ± 0.30 | 0.95 ± 0.07 |
1,500 cUMP | 3.6 ± 2.4 | 0.97 ± 0.04 |
15,000 cUMP | 11.7 ± 8.6 | 1.18 ± 0.17 |
Data were normalized to readings for cAMP alone and are presented as means ± SE, performed in triplicate. Indicated amounts of cNMP were added to known amounts of cAMP (4 or 150 pmol cAMP as indicated). Apparent cAMP levels were measured using cAMP enzyme immunoassays.