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| Channel 1: Nuclei | Value | |
|---|---|---|
| Dye/channel | Hoechst 33342 | Excite at 386 |
| Thresholding | Fixed intensity | 100 |
| Segmentation | Shape | 1 |
| Object.Area.Ch1 | Filters | Range within 2SD of mean for basal cells |
| Object.AvgIntensity.Ch1 | Filters | Range within 2SD of mean for basal cells |
| Channel 2: pATF2 or Ac-p65 | ||
| Dye/channel | Green | Excite at 485 |
| Thresholding | None | — |
| Selection | Circle around Ch1 | 10 |
| Region of interest ring Ch2 | Cytoplasm mask | Width 10, distance 0 |
| Region of interest circ Ch2 | Nuclear mask | 0 |
