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. 2015 Sep 4;21(21-22):2723–2732. doi: 10.1089/ten.tea.2015.0069

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© A. Aijaz et al. 2015; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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FIG. 5. — Quantification of keratinocyte scratch assays of cell migration and insulin bioactivity. (A) Rate of scratch closure following stimulation by media from empty microspheres (control) and insulin-containing conditioned media sampled from MR and μs RIN-m cells on days 1, 7, and 21 (μs-R1, μs-R7, and μs-R21, respectively). (B) Rate of scratch closure following stimulation by media from empty microspheres (control) and insulin-containing conditioned media sampled from MA and μs AtT-20ins cells on days 1, 7, and 21 (μs-A1, μs-A7, and μs-A21). Both cell types accelerated scratch closure, although conditioned media from RIN-m cells demonstrated a more profound effect on scratch closure than conditioned media from AtT-20ins. Asterisks indicate statistically significant differences (p < 0.05) between the indicated condition(s) and control. Error bars show standard error of mean.