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Concentration of insulin in insulin-conditioned media sampled from (A) MR and μs RIN-m cells on days 1, 7, and 21 (μs-R1, μs-R7, and μs-R21, respectively) and (B) MA and μs AtT-20ins cells on days 1, 7, and 21 (μs-A1, μs-A7, and μs-A21). Empty microspheres (control) served as insulin-free negative controls. Asterisks indicate statistically significant differences (p < 0.05) between the indicated condition(s) and control. Error bars show standard error of mean.
