FIG. 2.

Protection of mGluR4 activation on H₂O₂-induced NSC apoptosis. For estimating cell apoptosis, terminal deoxynucleotidyl transferase-mediated UTP nick end labeling (TUNEL) and propidium iodide–Hoechst (PI–Hoechst) staining were carried out after each treatment. NSC cultures were treated by the vehicle (Ctrl), 30 μM of VU0155041 (VU), 1 mM of mGluRs antagonist (RS)-a-methylserine-O-phosphate (MSOP), and MSOP plus VU0155041 (MSOP + VU) for 1 h, and then exposed to 100 μM of H₂O₂ for further 12 h. Representative images (A) and quantification (C) were shown after PI–Hoechst staining. Data from three independent experiments (n = 3) were presented as the percentage of PI-positive cells in the total Hoechst-positive cells. **P < 0.01 versus control, ^#P < 0.05 versus VU0155041 group. Scale bar 200 μm. Representative images and quantitative analysis for TUNEL staining were shown in (B) and (D), respectively. Data from three independent experiments (n = 3) were presented as the percentage of TUNEL-positive cells in total DAPI-stained cells. Scale bar 100 μm. *P < 0.05 versus control, ^#P < 0.05 versus VU0155041 group. Color images available online at www.liebertpub.com/scd