Table 1. Specific primers for real-time reverse transcriptase-polymerase chain reaction (RT-PCR) amplification, listed with their annealing temperature (A.T.).
| Gene Name | Fw primer | Rv primer | mRNA ID | A.T. (°C) |
|---|---|---|---|---|
| Rex1 | gtgcatcacacctcagactgt | cgttggttgaaggccaactg | NM_005106.4 | 61 |
| Oct4 | ctcctggagggccaggaatc | atatacacaggccgatgtgg | NM_00510 | 61 |
| Sox2 | ctccgggacatgatcagc | ggtagtgctgggacatgtgaa | NM_001109181.1 | 61 |
| Nanog | atgcctcacacggagactgt | aagtgggttgtttgcctttg | NM_005103.4 | 61 |
| Vinculin | aggagaccttgcgaagacagg | gcggttgccacttgtttag | NM_001107248 | 61 |
| HPRT | agccgaccggttctgtcat | agccgaccggttctgtca | NM_012583.2 | 61 |
Fw = forward; Rv = reverse.