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. 2015 Nov 19;5:16800. doi: 10.1038/srep16800

Table 1. Elution conditions of the HPLC for separation of the polySia fractions.

elution volume NH4HCO3
elution time NH4HCO3
in ml in % in minutes in %
0 30 0 30
110 50 440 50
115 100 460 100
125 100 500 100
130 0 520 0
160 0 640 0