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. 2015 Nov 19;5:16811. doi: 10.1038/srep16811

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Copyright © 2015, Macmillan Publishers Limited

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(a–e): Effect of Fra-2 silencing by specific siRNA in HPV^+ve and HPV^−ve TSCC cells. Representative immunoblots (Panel a,b) showing complete inhibition of Fra-2 expression at the concentration of 80 nM in both UPCI:SCC090;HPV16^+ve (a) and AW13516;HPV^−ve cells (b). 40 μg cellular proteins isolated from both the cell lines following treatment with Fra-2-siRNA (20 nM-80 nM) and scrambled control siRNA (80 nM) for 48 hours were examined for its effects on AP-1 family proteins (a,b), their target genes; Bcl-2, MMP-9 and CyclinD1 (c,d) and HPV16 E6/E7 (e). To ensure equal loading of protein, β-actin was used as an internal control. Quantitation of bands was performed by densitometric analysis. Significant (p < 0.05) when compared with untreated control vs. treated group. The data in bar diagram are expressed as the mean ± SD of three independent experiments. *p < 0.05 & **p < 0.01.