Table 2. Cumulative data of activation of AP-1 family proteins in HPV16+ve & HPV−ve tongue cancer cases as observed by band supershift assay.
| AP-1 family proteins(↓) | TSCC (n = 50) |
p-value | |||||||
|---|---|---|---|---|---|---|---|---|---|
| HPV16 positive TSCC (n = 14) |
HPV negative TSCC (n = 36) |
||||||||
| Nil - | Weak + | Moderate ++ | Strong ++++ | Nil - | Weak + | Moderate ++ | Strong ++++ | ||
| c-Jun | 14 | — | — | — | 7 | 9 | 20 | — | 0.0001 |
| JunB | 9 | 5 | — | — | 36 | — | — | — | 1.0 (ns) |
| JunD | 5 | 3 | 6 | — | 36 | — | — | — | 0.0001 |
| c-Fos | — | — | 2 | 12 | — | 1 | 6 | 29 | 1.0 (ns) |
| FosB | 14 | — | — | — | 36 | — | — | — | 1.0 (ns) |
| Fra-1 | 14 | — | — | — | 36 | — | — | — | 1.0 (ns) |
| Fra-2 | — | 2 | 3 | 9 | — | 2 | 2 | 32 | 0.31 (ns) |
#Data represent DNA binding activity of different AP-1 family proteins in >90% of cases with minor changes in few cases. Arbitrary level of DNA binding in band supershift assay: Nil/not detectable (0%, −); Weak (~25%, +); Moderate (~50%, ++); Strong (>80%, ++++). P-value obtained by probability Fisher’s (exact) test using Graph pad Instat (6.0) comparing the DNA binding of AP-1 proteins (nil+weak vs moderate+strong) among HPV16+ve cancer vs. HPV−ve cancer. P-values in bold (≤0.05) are considered as statistically significant. ns = not significant.