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. 2015 Jul 8;24(22):2687–2699. doi: 10.1089/scd.2015.0104

FIG. 3.

FIG. 3.

Effects of SHED-CM on DRG neuronal outgrowth and survival. (A) β-III-tubulin immunostaining of DRG neurons cultured with SHED-CM or DMEM (−) for 48 h. Scale bar = 100 μm. (B) Quantitative analysis of average neurite length. The average neurite outgrowth was significantly higher in neurons treated with SHED-CM than in those treated with DMEM (−). DMEM (−), SHED-CM: n = 5 per group. Data are shown as mean ± SD. *P < 0.05. (C) DRG neuron viability. Cell viability is reported as the absorbance at 450 nm following CCK-8 assay. Compared with DMEM (−), SHED-CM significantly increased the viability of DRG neurons. DMEM (−), SHED-CM: n = 5 per group. Data are shown as mean ± SD. *P < 0.05. DRG, dorsal root ganglion.