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. 2015 Jul 8;24(22):2687–2699. doi: 10.1089/scd.2015.0104

FIG. 4.

FIG. 4.

Effect of SHED-CM on capillary tube-like HUVEC formation. (A) HUVECs were cultured with EM, SHED-CM, VEGF (10 ng/mL), and HGF (10 ng/mL). After 11 days, capillary tube-like structures were observed. Scale bar = 100 μm. (B) Statistical analysis of the total blood vessel lengths. HUVECs cultured in SHED-CM exhibited significant increases in tube length when compared with cells cultured in DMEM (−). EM, SHED-CM, VEGF, HGF: n = 3 per group. Data are shown as mean ± SD. *P < 0.05. (C) Statistical analysis of the numbers of joints. HUVECs cultured in SHED-CM exhibited significantly higher numbers of joints when compared with cells cultured in DMEM (−). EM, SHED-CM, VEGF, HGF: n = 3 per group. Data are shown as mean ± SD. *P < 0.05. EM, endothelial cell medium; HGF, hepatocyte growth factor; HUVEC, human umbilical vein endothelial cells; VEGF, vascular endothelial growth factor.