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. 2015 Nov 19;5:16978. doi: 10.1038/srep16978

Figure 4. Cep55 knockdown causes increase of cyclin B1 level, non-segregation of homologous chromosomes, and failure of PB1 extrusion.

Figure 4

After microinjection of Cep55 siRNA or control siRNA, the oocytes were placed in M16 containing IBMX for 24 h, then washed 8 times and transferred into IBMX-free M16 medium for 14 h to observe meiotic cell cycle progression. (A) Percentages of first polar body extrusion in the knockdown group and control group. Data are presented as means ± SEM of 4 independent experiments (*p < 0.01). (B) Failure of homologous chromosome segregation after Cep55 knockdown. The ratio of tetrads is 35/44 in the Cep55 knockdown and 7/41 in the control group. (C) Higher cyclin B1 level in Cep55 knockdown oocytes. Oocytes of the Cep55 knockdown group and control group were cultured in IBMX-free medium for 9.5 h, followed by western blotting. (D) The relative expression level of cyclin B1 after Cep55 knockdown. Data are presented as means + -SEM of 3 independent experiments (p < 0.05).