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After microinjection of Cep55 siRNA or control siRNA, the oocytes were placed in M16 containing IBMX for 24 h, then washed 9 times and transferred into IBMX-free M16 medium for 9.5 h. Bub3 (green) was localized at the kinetochores in the Cep55 si-group, while in the control group, Bub3 was dissociated from kinetochores of separating homologous chromosomes (red).
