FIG. 9.

Ligamentous differentiation live immunostaining assay. The stem cells were cultured on scaffolds in the presence of FBS only, PL, fibroblast growth factor (FGF) in FBS (FGF), or PL and FGF. The positive control was anterior cruciate ligaments (ACL) fibroblasts. The red staining represents cells expressing the different marker ligamentous differentiation markers. The blue staining represents the live cells covering the scaffolds (DAPI nuclear staining). Color images available online at www.liebertpub.com/tea