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. 2015 Nov 19;5:16883. doi: 10.1038/srep16883

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(A,B) HIPS-AF647 fluorophore-labeling and purification of EcMutS-his₆/ald₆. Final heparin chromatography (A) and Fluorescence scan (top) and coomassie stain (bottom) of eluted fractions separated using SDS-PAGE gel (B). (C,D) HIPS-AF555 fluorophore-labeling and purification of EcMutS-his₆/ald₆(D835R,R840E). Final heparin chromatography (C) and Fluorescence scan (top) and coomassie stain (bottom) of eluted fractions separated using SDS-PAGE gel (D). (E,F) HIPS-AF647 fluorophore-labeling and purification of EcMutL-his₆/ald₆. Fluorescence scan (top) and coomassie stain (bottom) of eluted fractions separated using SDS-PAGE gel (E) and fluorescence scan (left) and coomassie stain (right) with Gaussian fitting of labeled (red) and unlabeled (green) EcMutL-his₆/ald₆ (F). The Coomassie stained gels have been cropped to show only the relevant protein bands, which in these studies accounts for >90% of the visible bands.