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. 2015 Nov 19;5:16883. doi: 10.1038/srep16883

Table 1. DNA Binding, Dissociation and ATP Processing Constants for E.coli MutS.

Protein kon (105 × M−1 × sec−1) koff (10−4 × sec−1) KD (nM) koff·ATP (sec−1)
AF555 labeled EcMutS- his6/ald6(D835R, R840E) 4.37 ± 0.90 24.48 ± 1.44 5.75 ± 1.51 0.45 ± 0.09
Unlabeled EcMutS- his6/ald6 (D835R, R840E) 9.48 ± 3.90 33.02 ± 1.87 3.85 ± 1.78 0.48 ± 0.09
AF647 labeled EcMutS- his6/ald6 3.44 ± 0.52 8.93 ± 0.34 2.63 ± 0.49 0.26 ± 0.08
Unlabeled EcMutS- his6/ald6 5.29 ± 1.82 6.10 ± 0.53 1.25 ± 0.53 0.53 ± 0.11