Table 1.
Plackett-Burman 11/12 screening design: two levels, 6 factors, 3 central points, and 5 dummy factors (D1, D2, D3, D4, and D5).
| Run | Temperature (°C) | % biomass (w/v) | pH | Cellulase from PO (U/gds)a | Cellulase from Trichoderma reesei ATCC26921 (U/gds) | Time (hours) | Dummy 1 | Dummy 2 | Dummy 3 | Dummy 4 | Dummy 5 | Total sugars released (mg/gds) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 45 | 7 | 3 | 10 | 1.8 | 24 | 1 | 1 | 1 | −1 | 1 | 179.15 |
| 2 | 45 | 3 | 6 | 30 | 1.8 | 24 | −1 | 1 | 1 | 1 | −1 | 509.95 |
| 3 | 25 | 7 | 6 | 10 | 5.4 | 24 | −1 | −1 | 1 | 1 | 1 | 173.70 |
| 4 | 45 | 7 | 3 | 30 | 1.8 | 72 | −1 | −1 | −1 | 1 | 1 | 408.55 |
| 5 | 45 | 3 | 6 | 30 | 5.4 | 24 | 1 | −1 | −1 | −1 | 1 | 439.30 |
| 6 | 45 | 7 | 6 | 10 | 5.4 | 72 | −1 | 1 | −1 | −1 | −1 | 312.75 |
| 7 | 25 | 7 | 6 | 30 | 1.8 | 72 | 1 | −1 | 1 | −1 | −1 | 112.15 |
| 8 | 25 | 7 | 3 | 30 | 5.4 | 24 | 1 | 1 | −1 | 1 | −1 | 344.35 |
| 9 | 25 | 3 | 3 | 30 | 5.4 | 72 | −1 | 1 | 1 | −1 | 1 | 461.70 |
| 10 | 45 | 3 | 3 | 10 | 5.4 | 72 | 1 | −1 | 1 | 1 | −1 | 598.30 |
| 11 | 25 | 3 | 6 | 10 | 1.8 | 72 | 1 | 1 | −1 | 1 | 1 | 456.35 |
| 12 | 25 | 3 | 3 | 10 | 1.8 | 24 | −1 | −1 | −1 | −1 | −1 | 271.60 |
| 12C | 35 | 5 | 4.5 | 20 | 3.6 | 48 | 0 | 0 | 0 | 0 | 0 | 387.40 |
| 14C | 35 | 5 | 4.5 | 20 | 3.6 | 48 | 0 | 0 | 0 | 0 | 0 | 419.30 |
| 15C | 35 | 5 | 4.5 | 20 | 3.6 | 48 | 0 | 0 | 0 | 0 | 0 | 355.50 |
aDue to the copresence of two (hemi)cellulolytic activities in the P. ostreatus supernatant, when 10, 20, and 30 U/gds of cellulase are utilized in the different experimental runs, the xylanase concentrations in the enzymatic hydrolytic mixtures are 200, 400, and 600 U/gds, respectively.