Table 1.

Properties of RNA-seq libraries

Library	Library preparation				Sequencing
	RNA source	Duration of RNA fragmentation (min)	× AMPure volume (targeted fraction to retain)	PCR cycles
A	-4 dpo whole embryoa	8	× 1.6 (>100 bp)	6	HiSeq 1/4 lanes, 171 cycles paired-end
B		2	× 0.7 (>300 bp)	6
C				6	MiSeq 1/4 lanes, 250 cycles paired-end
D	9 dpo whole embryo	8	× 1.6 (>100 bp)	2	HiSeq 1/4 lanes, 171 cycles paired-end
E		2	× 0.7 (>300 bp)	4
F	30 dpo head	4	× 1.0 (>150 bp)	6	HiSeq 2/3 lanes, 151 cycles paired-end
G	30 dpo liver			6
H	30 dpo tail			6

^aEmbryo of 4 days before the estimated day of oviposition