Figure 2.
3 H-thymidine uptake assay and PKH staining of circulating PBMC from (un)infested Belgian Blue and Holstein–Friesian cattle. A Mean stimulation index (SI ± SEM) of PBMC from uninfested (n = 8) and infested (n = 12) BB cattle re-stimulated with medium, 4 different concentrations of P. ovis and ConA. B Mean stimulation index (SI ± SEM) of PBMC from uninfested (n = 4) and infested (n = 4) HF cattle re-stimulated with medium, four different concentrations of P. ovis and ConA. C Mean proliferation index (PI ± SEM) of PKH labelled PBMC re-stimulated with 5 μg/mL P. ovis antigen compared to unstimulated PBMC (dashed horizontal line) of infested BB (n = 12). D Mean proliferation index (PI ± SEM) of PKH labelled PBMC re-stimulated with 5 μg/mL P. ovis antigen compared to unstimulated PBMC (dashed horizontal line) of infested HF (n = 4). *P < 0.05, (*) P = 0.057.