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. 2015 Nov 18;16:971. doi: 10.1186/s12864-015-2130-z

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© Liu et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Phenotypic masculinization and their gonadal morphology in the germ cell-depleted adults. a-e, f-j and k-o Representative images of control gynogenetic females, control males from sexual reproduction, and the germ cell-depleted gynogenetic males, respectively. a, f and k Body shape. b, g and l Gill cover. c, h and m Anus. d, i and n Images of mature ovary, testis, and 1-year-old germ cell-depleted gonad, respectively. e, j, o HE staining of control ovary, control testis, and 1-year-old germ cell-depleted gonad, respectively. Green circle indicates the cavity, inset shows the whole cross section of 1-year-old germ cell-depleted gonad. Pm, Peritoneal membrane; Sg, spermatogonia; Sc, spermatocytes; Sz, spermatozoa; L, Leydig cells; S, Sertoli cells; My, peritubular myoid cells. [Scale bars, D, I and N, 2 cm; E 100, μm, J and O 50 μm]