Fig. 6.

Gonadal structures and gene expression patterns in adult germ cell-depleted gynogenetic gonads. a-d The external morphology of 1 year to 3 years old germ cell-depleted gonads. a 1-year-old germ cell-depleted gonads. b 2-year-old germ cell-depleted gonads. c and d 3-year-old germ cell-depleted gonads. e-h Cross section of germ cell-depleted gonads in above box 1 (e), 2 (f), 3 (g) and 4 (h). Green arrow indicated the cavity. L, Leydig cells; S, Sertoli cells. [Scale bars, 2 cm (a-d) and 50 μm (e-h). i RT-PCR analysis of somatic gene expression in control testis (Con-testis) from sexual reproduction, control ovary (Con-ovary) and the 3-year-old germ cell-depleted gonad (Morphant gonad). The gene expression level is related to β-actin and Rpl13a