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Reactivities of UB-mimicking peptides with E1 and E2 enzymes. (A) Transfer of biotin-labeled peptides to the E1 enzyme Ube1. Biotin-labeled UB was used as a control in the reactions. (B) Transfer of biotin-labeled peptides P2, P3 and P4 from E1 to the E2 enzymes Ubc1, UbcH5a and UbcH7.
