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Kinetic parameters of ATP-PPi exchange catalyzed by Ube1 with the UB-mimicking peptides.
| K1/2 (µM) |
kcat (min−1) |
kcat/K1/2 (µM−1 min−1) |
|
|---|---|---|---|
| wtUB (full length) | 1.4 ± 0.5 | 88 ± 17 | 60 |
| C-terminal peptides of wtUB and variants | |||
| P1 (70VLRLRGG76) | -a | -a | 7.7 × 10−5 |
| P2 (70VWRFHGG76) | 342 ± 17 | 3.5 ± 0.6 | 1.0 × 10−2 |
| P3 (70VQRYWGG76) | 426 ± 11 | 9.7 ± 1.4 | 2.3 × 10−2 |
| P4 (70VYRFYGG76) | 141 ± 5 | 15 ± 2.7 | 1.1 × 10−2 |
K1/2 and kcat could not be determined for P1 due to its low activity.
