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. 2015 Oct 16;10(10):e0140252. doi: 10.1371/journal.pone.0140252

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© 2015 Baldan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — a-e) histochemical analysis of 8 day-after-inoculation (dai) Arabidopsis DR5::GUS reporter plantlets. A strong induction of β-glucuronidase activity (blue staining) is appreciable in all treated samples (c-e) and in the IAA-treated seedlings (IAA 50 nM, b) compared to non-inoculated seedlings (a). f) 4MU-fluorimetric assay on total protein extract of 8-dai Arabidopsis seedlings highlighted a strong induction of β-glucuronidase activity both in the whole IAA and GL83 treated-plantlets. Data were normalized on non-treated seedlings (CTRL) and reported as percentage of the CTRL. Statistically significant at *, P< 0.01.