Fig. 8.

A) Flinn diagram displaying lacunar shapes in WT and Phospho1 KO tibia at tibia–fibula junction from various ages. The x axis represents lacunar flatness which was calculated by dividing lacunar intermediate radius (l2: length of best-fit ellipsoid's intermediate radius) with lacunar minor radius (l3: length of best-fit ellipsoid's minor radius). The y axis represents lacunar elongation which was calculated by dividing lacunar major radius (l1: length of best-fit ellipsoid's major radius) with lacunar intermediate radius (l2: length of best-fit ellipsoid's intermediate radius). Data represent means with group sizes of n = 4 for WT and KO mice from different ages. B) Surface representation of the lacunar (yellow) and red (vascular porosity) segmented from 300 consecutive images from tibia–fibula junction from both genotypes and each age.