FIG. 4.

Venn diagram comparing the number of proteins identified in ULF from cyclic (n = 4) and confirmed pregnant (n = 4) heifers on Day 16 (Day 0 = estrus) as well as Day 16 conceptus-conditioned cultured medium after culture for 6 h (A) or 24 h (B). Proteins were determined by total peptide counts following nano liquid chromatography tandem mass spectrometry (nano-LC-MS/MS) and had to be present in at least three of four heifers in each group. The 30 proteins listed were those identified only in pregnant ULF (i.e., not cyclic) on Day 16 and in spent culture medium. Full details of all the proteins are listed in Supplemental Tables S3 and S4.