Figure 1.

(A) Outline of the experimental design and workflow for evaluating different combinations of alignment tools and normalization procedures. (B) Comparison of alignment tools. miRNA counts recovered from the different aligners were averaged across the samples and plotted for each pairwise comparison. Only miRNAs called present by all the aligners were considered. The aligners being compared are shown on the diagonal, along with the distinct number of miRNAs identified after filtering for species with low counts. Spearman’s correlation coefficients are indicated in the upper left corner for each comparison.